No, generally speaking your own DNA already contains the information required to carry out this test. In the case of males both maternal and paternal ancestry can be determined from your DNA sample. If you are female, your sample will only have ancestry information related to the maternal line, therefore your brother or your father will be required to contribute a sample if you want to know more about your paternal lineage. Please note that a full brother will also have the same mtDNA as you, so his sample can be used directly for a dual ancestry test on your behalf.

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